Question

to observe root hair on the surface of roots. For assessment.Also diagram.​

Answer 1

Answer:

The capacity of plants to absorb both water and mineral nutrients from the soil is related to the plant´s ability to develop an extensive and well-located root system. Of the total root surface area, root hairs can contribute up to 67 %. (Nielson et al., 2001). For determination of root hair length and density, Miguel (2004) stained roots of 14-days old plants that were observed under microscopy at 40x magnification. Using a comparative picture taken at the same magnification with micrometer scale, actual root dimensions were determined. Pictures were taken using a digital camera attached to dissecting scope and saved using Adobe Photoshop 7.0 software. Then the pictures were transferred to the Scion Image 1.63 software for root length determination at that magnification. For root hair density, a known surface area at that magnification was selected and the number of root hairs in the selected area was determined. After having root hair measurements (length and density) further calculations were made to convert to real root hair length in millimeters, and root hair density in number of root hairs per square millimeter. These calculations were made using as inputs, the picture magnification, micrometer scale and conversion of the selected root surface area to the unit of root surface area in square millimeters. Thus, root hair length (mm) and root hair density (number of root hair per mm 2 of root surface area) was determined for basal and lateral roots. This method is very laborious in a breeding program where a large number of genotypes are evaluated. The objective of this research was to test a simple method of root hair evaluation.

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