Question

To observe stomata in a plant cell why is the peel taken from the lower surface of the leaf

Answer 1

Pluck a fresh leaf from a balsam plant.

Fold the leaf and carefully tear along the bruised area of the lower side of the leaf.

We can see a colourless narrow border along the torn edge.

Carefully pull out the thin membranous transparent layer from the lower epidermis using a forceps.

Put the epidermis into a watch glass containing distilled water.

Take few drops of Safranin solution using a dropper and transfer this into another watch glass.

Using a brush transfer the epidermis into the watch glass containing the Safranin solution.

Keep the epidermis for 30 sec in the Safranin solution to stain the peel.