Question

to prepare a temporary mount of a leaf peel to study the structure of stomata

Answer 1

Aim: To prepare a temporary amount of leaf to study structure of stomata.

Materials Required: A leaf, forceps, needles, watch glass, glass slides, droppers, coverslips, blotting paper, safranin, brush, glycerine and a compound microscope.

Procedure:

• Pluck  a healthy leaf from a potted plant.
• Remove peel from lower part of leaf by forceps as the lower part of peel contains maximum stomata. Keep the peel in watch flass containing water so that epidermal cells do not get dry.
• then put a few drops of safranin stain in watch glass.
• After 2-3 minutes take out the peel and place it on a clean glass slide.
• Put a drop of glycerin over the peel and gently cover it with a clean coverslip with the help of a needle.
• Remove extra stain and glycerin by using blotting paper.
•  Now, observe the peel under a compound microscope.

Observations:

• The epidermal cells are visible. These are irregular in outline and have no intracellular spaces.
• Many small pores are seen scattered among the epidermal cells.
• Each pores are guarded by two bean- shape like structure, each containing chloroplast and nucleus called stomata.
• The stomata may be opened or closed. The guard cells regulates the opening and closing of stomata.

Result:

Stomata are present on epidermal cells of the leaf.

Precautions:

• Cut the peel to a proper size and avoid folding it.
• Always place the peel at the centre of the slide and hold it from edges.
• Do not put too much of stain on the leaf peel.
• Always handle peel with brush as needle may damage the cells present on peel.
• Place coverslip gently avoiding entry of any bubbles.
• Remove Extra stain by using a blotting paper.

Thank you .

Answer 2

$\huge \red {\underline {HAYE\:MATE}}$

$\huge \red {\mathbb {ANSWER}}$

The steps are:

i. Removal of peel from leaf.

ii. Stain with safranin.

iii. Put the stained peel on a clean slide.

iv. Mount it with glycerine and cover slip.

$\huge \blue {\mathbb {THANKS}}$

$\huge \green {FOllOW\:ME}$

$\huge \purple {\mathbb {SINGLE\:STAR}}$