Question

To prevent detachment of collagen from plastic substrate, it was ______ to the plastic using carbodiimide

Answer 1

Answer:

C2c12 on cover slips are coated with either different plates coated with a poly lysine, fibron nectin

Answer 2

To prevent detachment of collagen from plastic substrate, protein coating is done to the plastic using carbodiimide.

Explanation:

• Many experiments conducted in the laboratory have a problem of sticking collagen to the plastic substrate.
• This interferes with the further process of tissue culture.
• Plastic plates are replaced either by glass plates or it has been coated with protein like native or denatured collagen, fibronectin, laminin, and vitronectin.
• This process is known as the cross-linking of protein in the process of Human Mesenchymal Stem Cells tissue culture.
• Carbodiimide is an agent that is used to successfully implement the cross-linking process.

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To prevent detachment of collagen from plastic substrate, it was............ to the plastic using carbodimide .

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