Question

to prove that co2 is released during anaerobic repiration what are the precautions should be taken to this activity​

Answer 1

PRECAUTIONS:

1.Germinating seeds should be kept moist.

2.All connections of the set-up should be air-tight.

3.Freshly prepared KOH solution should be used.

4.Keep one end of U-shaped delivery tube in conical flask and the other end immersed in water of the beaker.

5.The test tube containing KOH should be hung carefully.

Explanation:

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Answer 2

Precautions to be taken to prove that CO₂ is released during anaerobic respiration

Explanation:

Principle:

In this experiment, moistened gram seeds are used. So that they release carbon-di-oxide and anaerobic respiration occurs. Anaerobic respiration is nothing but it lacks oxygen. KOH solution consumes the released carbon-di-oxide.

Method

• Germinate seeds by wrapping them in moist blotting paper or cotton wool for around 3 to 4 days.

• Place the germinated seeds in a conical flask and some water is sprayed into the flask. Use the thread to suspend the conical flask that has the test tube with 20% KOH solution. The rubber cork is used to seal the flask opening.

• In this case,  insert one edge of the U-shaped glass delivery tube through the hole in the rubber cork and one edge into a beaker saturated with water. It is important to note that all the attachments are sealed.
• It is necessary to mark the initial level of water in the U-shaped delivery tube.
• The experiment is left uninterrupted for 1 to 2 hours to observe the water fluctuation in the test tube.

Observation and Conclusion :

• During the respiration process in the conical flask, carbon dioxide is released from the germinating gram seeds, which indicates the rising level of the water.
• The KOH solution suspended in the test tube in the conical flask absorbs or consumes the carbon dioxide released during the process, resulting in a vacuum or void in the flask and upward water movement in the test tube. As a result, the water level in the test tube fluctuates.

Precautions

• Moisturizing seeds before germinating them is necessary

• All connections in the experimental arrangement must be kept in an airtight environment.

• The KOH solution used must be freshly prepared.

• One end of the delivery tube must be kept in the conical flask. The beaker's other edge is submerged in water.

• The tube containing the KOH solution must be properly suspended.