To prove that product of fermentation is carbon dioxide in procedure, observation and conclusion....
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The rise in the level of water indicates that CO2 is produced by germinating seeds during respiration. Actually, the germinating seeds respire and produce CO2, which is absorbed by KOH solution. This creates a vacuum in the conical flask. The air present in the bent glass tube moves into the conical flask.
Fermentation reacts NADH with an endogenous, organic electron acceptor. Usually this is pyruvate formed from sugar through glycolysis. The reaction produces NAD+ and an organic product, typical examples being ethanol, lactic acid, and hydrogen gas (H2), and often also carbon dioxide.
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