To show chloropyll is esssential for phosynthesis
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Role of Chlorophyll in Photosynthesis
The overall balanced equation for photosynthesis is:
6 CO2 + 6 H2O → C6H12O6 + 6 O2
where carbon dioxide and water react to produce glucose and oxygen. However, the overall reaction doesn't indicate the complexity of the chemical reactions or the molecules that are involved.
Plants and other photosynthetic organisms use chlorophyll to absorb light (usually solar energy) and convert it into chemical energy. Chlorophyll strongly absorbs blue light and also some red light. It poorly absorbs green (reflects it), which is why chlorophyll-rich leaves and algae appear green.
In plants, chlorophyll surrounds photosystems in the thylakoid membrane of organelles called chloroplasts, which are concentrated in the leaves of plants. Chlorophyll absorbs light and uses resonance energy transfer to energize reaction centers in photosystem I and photosystem II. This happens when energy from a photon (light) removes an electron from chlorophyll in reaction center P680 of photosystem II. The high energy electron enters an electron transport chain. P700 of photosystem I works with photosystem II, although the source of electrons in this chlorophyll molecule can vary.
Electrons that enter the electron transport chain are used to pump hydrogen ions (H+) across the thylakoid membrane of the chloroplast. The chemiosmotic potential is used to produce the energy molecule ATP and to reduce NADP+ to NADPH. NADPH, in turn, is used to reduce carbon dioxide (CO2) into sugars, such as glucose
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