Question

Topic - virology

Q. how to calculate if an inhibitor "X" has an effect on the virus entry in cell culture?

Answer 1

Explanation:

I am trying to determine if an inhibitor "X" is blocking the early events in a virus "Y". The cell culture, incubation with the inhibitor and the virus infection are standarized in the lab. I run a control in which the cells are non-treated with the inhibitor and infected as usual to see the effect by flow cytometry (The virus code GFP after the entry in the cells).

Answer 2

Answer:

I am trying to determine if an inhibitor "X" is blocking the early events in a virus "Y". The cell culture, incubation with the inhibitor and the virus infection are standarized in the lab. I run a control in which the cells are non-treated with the inhibitor and infected as usual to see the effect by flow cytometry (The virus code GFP after the entry in the cells). My question is: what is the best mathematical approach to calculate the effect of the inhibitor "X" on the virus entry?. I am doing a simple "rule of three" where the virus infection in presence of inhibitor is taken as the 100%. Is there another way to estimate that?

Explanation:

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