Transgenic crop that have long shelf biology discussion
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The application of recombinant technique for horizontal transfer of gene in plant system has several limitations. Plant genome is exceedingly complex, involving a huge amount of DNA, which can accommodate sequences equivalent to 6-7 million genes in an average crop plant. Several genes are located outside the nucleus, namely the chloroplastids and mitochondria.
Moreover, every gene, in any system, has its promoter, structural sequences and terminator—all of which require to be identified before introduction. For instance, the nitrogen fixing gene of bacteria—Rhizobium has 17 genes of 24 kilo-bases, distributed under eight operational units or operons.
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