Question

Transgenic plants * 1 point contain foreign genes in their cells are wildtype plants are plants that differ in geographical locations lack transmembrane proteins Type II restriction enzymes cut the sequence in the following way * 1 point Within the recognition sequence At 100-1000 nucleotides away from the recognition sequence At 27-30 nucleotides away from the recognition sequence It cuts randomly Which of the following bacterium is considered as “Natural Genetic Engineer”? * 1 point Agrobacterium tumefaciens Agrobacterium radiobactor Pseudomonas putida E. coli Which of the following is supplemented with vitamin A in order to improve its nutritional quality? * 1 point cotton potato tomato rice Name the enzyme which has silenced to delay the fruit ripening process? * 1 point Polygalacturonase Glyphosate ligase P53 The particle gun method consists of which of the following steps? * 1 point The DNA of interest is absorbed onto microprojectile beads These beads are often made of aluminium The explosion in a gun propels a macroprojectile forward which in turn propels microprojectile beads The macroprojectiles and microprojectiles both cross the perforated plate and hit the target tissue behind it Name the technique which is used to enhance the life of a tomato * 1 point Antisense technology transduction Ex vivo gene transfer Molecular farming A DNA strand with the sequence 5’CGA TTG 3’would be complementary to the sequence * 1 point 5’GCU AAC 3’ 5’ GCT AAC 3’ 5′ GTT AGC 3′ 5′ CAA TCG 3′ Insect resistance in the transgenic plant has been achieved by * 1 point transferring genes for Bt toxins transferring genes for protease inhibitors transferring genes for other insecticidal secondary metabolities all of the above Which of the following techniques could be used to demonstrate protein binding to specific DNA sequences? * 1 point Western blot hybridization Northern blot hybridization Southern blot hybridization Electrophoretic mobility shift assay Which enzyme is used to join nicks in the DNA strand? * 1 point Primase DNA polymerase DNA ligase Endonuclease Resistance to glyphosphate in transgenic petunia plants has been developed by the transfer of * 1 point gene for EPSPS(5-enol-pyruvyl shikimate 3 phosphate synthase) gene for ALS (acetolactate synthase) gene for GS (glutamine synthase) all the above What technique can be used to introduce genetic materials into plant cells? * 1 point GFP Gene gun Fixation Staining What does “Ti” stand for in Ti plasmid? 1 point Titanium Tumor inducing Tuberculosis inducing None of the above The process of invitro amplification of specific DNA sequences by an enzymatic process is termed as * 1 point synthesis polymerase chain reaction (PCR) translation microarrays Which PCR variation allows a scientist to immediately quantify the results? * 1 point Real-time PCR Nested PCR Multiplex PCR Reverse transcriptase PCR Cry genes or Bt genes are obtained from * 1 point Cotton pest Tobacco plant Bacillus thuringiensis E. coli What is Gene * 1 point The segment of RNA codon Polypeptide chain The segment of DNA Which enzyme is useful in genetic engineering? * 1 point lactase Amylase Lipase Restriction endonuclease Introduction of DNA into cells by exposing to high voltage electric pulse is * 1 point eletrolyis electroporation electrofusion electrofision

Answer 1

What the hell is this ?