Chemistry, asked by Suraj9423, 1 year ago

Triple sugar iron test procedure for novel isolates

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Answered by Anonymous
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TSI Agar is used for the determination of carbohydrate fermentation and hydrogen sulfide production in the identification of gram-negative bacilli.1,2 Hajna developed the formulation for TSI Agar by adding sucrose to the double sugar (dextrose and lactose) formulation of Kligler Iron Agar.3 The addition of sucrose

Answered by AfreenMohammedi
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Answer:

Principle. The triple sugar- iron agar test employing Triple Sugar Iron Agar is designed to differentiate among organisms based on the differences in carbohydrate fermentation patterns and hydrogen sulfide production. ... Due to the building of acid during fermentation, the pH falls.

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