Two sister chromatids of each chromosome separate from each other in. Anaphase - 1, Telophase -1 , Diakinesis , pachytene.
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Metaphase leads to anaphase, during which each chromosome's sister chromatids separate and move to opposite poles of the cell. Enzymatic breakdown of cohesin — which linked the sister chromatids together during prophase — causes this separation to occur.
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