Question

Types of restriction endonucleases biology discussion

Answer 1

Restriction enzymes, also known as restriction endonucleases, have played a key role in the development of recombinant DNA technology. These have been found in microorganisms tested and are known to cut double-stranded DNA to yield restriction fragments.

The first observations on the existence of restriction enzymes was made by Arber and Dussoix in 1962, and proposed model to explain the restriction phenomenon. Their views on restriction enzymes affirmed that certain bacterial strains contained endonucleases able to cleave unprotected DNA. In addition, several other strains contained a modification system responsible for protecting their own DNA.

Answer 2

Function of Restriction Endonucleases

Explanation:

• The restriction endonucleases are ordered into four group (Types I, II III, and IV) in their organization and catalyst cofactor prerequisites, the nature of their objective arrangement, and the situation of their DNA cleavage site comparative with the target sequence
• Type II enzymes cut DNA at characterized positions near or inside their recognition sequences
• They produce discrete limitation sections and particular gel banding examples, and they are the main class utilized in the research center for routine DNA examination and gene cloning