Question

under what circumstances could a lysate of Phage P1 containing transducing particles carry lambda lysogen ??​

Answer 1

Answer:

P1 is a temperate bacteriophage that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle the phage genome exists as a plasmid in the bacterium[1] unlike other phages (e.g. the lambda phage) that integrate into the host DNA. P1 has an icosahedral head containing the DNA attached to a contractile tail with six tail fibers. The P1 phage has gained research interest because it can be used to transfer DNA from one bacterial cell to another in a process known as transduction. As it replicates during its lytic cycle it captures fragments of the host chromosome. If the resulting viral particles are used to infect a different host the captured DNA fragments can be integrated into the new host's genome. This method of in vivo genetic engineering was widely used for many years and is still used today, though to a lesser extent. P1 can also be used to create the P1-derived artificial chromosome cloning vector which can carry relatively large fragments of DNA. P1 encodes a site-specific recombinase, Cre, that is widely used to carry out cell-specific or time-specific DNA recombination by flanking the target DNA with loxP sites (see Cre-Lox recombination).

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Answer 2

Answer:

Transduction is the transfer of DNA from one bacterium to another by a phage. ... Specialized transducing phages are phage that have a segment of bacterial DNA integrated into their genome. An example of a specialized transducing phage is lambda dgal, which carries the gal operon from E. coli.

Lysogenic Infection. MOSTLY UNAMBIGUOUSLY USED TERM. A reductive infection that results in ongoing phage genome replication, as a prophage, and specifically does not involve virion production except following subsequent prophage induction.

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