Under which of the following condition a Gram positive bacteria turns Gram negative
A) Defective staining technique
B) Changed pH of medium
C) Aged bacterial culture
D) All the above
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The answer is A Defective staining technique
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The condition when a Gram-positive bacteria turns Gram-negative is C) Aged bacterial culture.
- Gram-positive and gram-negative bacteria are defined based on the thickness of the peptidoglycan layer of the cell wall
- When a gram-positive bacteria become old then the peptidoglycan layer will also become thin then the gram-positive bacteria turns into either a gram-negative bacteria or gram variable bacteria
- Gram-negative bacteria have an outer lipid membrane while gram-positive bacteria do not have it that is why gram-negative bacteria are harder to kill.
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