Question

Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant vector molecule cannot be created.
(a) How are the desirable DNA sequences cut ?
(b) Explain the technique used to separate the cut fragments.
(c) How are the resultant fragments joined to the vector DNA molecule ?

Answer 1

Answer:

Explanation:

a. desirable DNA is cut with the help of Restriction enzyme(molecular scissors){fact 1'st RE found was Hind III)

b.Gel electrophoresis in this technique dna fragment is embedded in the well containing agarose gel b/w cathode and anode the cathode repulses the dna fragment as both are -vely charged and the anode attracts the dna fragment as anode is +vely charged, the smaller the fragment farther it moves .

then this gel pieces  are collected with help of spooling(process called elution) and then it is stained with ethidium bromide and observed under uv light under which DNA fragments are seen to be bright orange colored.

c.these are joined with the help of DNA ligase(molecular Glue).