Question

Uptake and utilization of amino acids by resting cells of nitrosomonas europaea.

Answer 1

Incorporation of selected amino acids by resting cells was studied with regard to effects of concentration, rate and extent of incorporation, cellular distribution, effect of ammonium ion on uptake, and competitive effects. l-Aspartic acid, l-alanine, l-serine, and glycine presented at trace levels were incorporated at rates ranging from 0.11 to 8.2 μμmoles per mg (dry weight) per minute, and maximal incorporation was 11 to 333 μμmoles per mg (dry weight). When glycine and aspartic acid were supplied at substrate level, the rate of incorporation increased 14- and 109-fold, respectively. The presence of ammonium ion further increased both the rate and extent of uptake of glycine and aspartic acid. The distribution of cellular radioactivity arising from 14C amino acids indicated that cell pool radioactivity was concentrated from 1.2- to 24.5-fold over the external medium. 

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Answer 2

Answer:

Clark C, Schmidt EL (1967b) Uptake and utilisation of amino acids by resting cells of Nitrosomonas europaea. J Bacteriol 93: 1309– 1315.