UV/Vis Spectrometry is used frequently for the quantitative analysis of biochemically important molecules, such as reduced nicotinamide-adenine dinucleotide (NADH) and cystidine monophosphate (CMP). A 56.0 mM solution of NADH has an absorbance of 0.285 at a wavelength of 340 nm and 0.683 at 260 nm, while an equimolar solution of CMP has an absorbance of 0.647 at 260 nm and an absorbance at 340nm that is indistinguishable from the reagent blank. A sample might contain NADH only, CMP only or a mixture of both NADH and CMP, gives an absorbance of 0.371 at 340 nm and 1.50 at 260 nm. Which species are present and at what concentration?
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