Question

vectors,
Explain the features of cloning​

Answer 1

Answer:

Vectors are used in science to describe anything that has both a direction and a magnitude. They are usually drawn as pointed arrows, the length of which represents the vector's magnitude.

A vector is an object that has both a magnitude and a direction. Geometrically, we can picture a vector as a directed line segment, whose length is the magnitude of the vector and with an arrow indicating the direction. ... Two examples of vectors are those that represent force and velocity.

Explanation:

The plasmids are the small extra-chromosomal DNA present in the bacteria which are commonly used as a cloning vector. The desired gene fragment can be inserted in the plasmid and then introduced into the host. The plasmid is selected on the basis of certain features.

   The plasmid can be isolated from the bacterial cells very easily.

   There should be single restriction sites for the restriction enzyme. This would allow proper integration of the gene without disintegration of the plasmid.

   The molecular weight of the plasmid should be high which would allow easy separation of the plasmid from the cellular debris.

   The copy number of the plasmid should be high in order to obtain huge amount of products from the gene.

   The plasmids and the bacteriophage DNA have the ori site. This is the site which helps in the initiation of replication.

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Answer 2

Answer:

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