Question

vi)
Give an
example of a plasmid vector used in cloning.​

Answer 1

Answer:

Escherichia coli and cloning vectors in E.coli include plasmid,bacteriophages ( such as phage) cosmids and bacterial artificial chromosomes (BACs)

Answer 2

pBR322

A cloning vector must have a replication origin to self-replicate inside the host cell.  Cloning vectors come in a variety of shapes and sizes.

• Plasmid vector
• Bacteriophage vector
• Phagemids
• Bacterial artificial chromosome
• Yeast artificial chromosome
• Cosmids
• Retroviral vector
• Human Artificial chromosome

Plasmid vector:

• The earliest vectors used in gene cloning were these.
• Bacteria, eukaryotes, and archaea all have them.
• These are self-replicating, extrachromosomal DNA molecules.
• They have a large number of copies and genes that make them resistant to antibiotics.
• They produce proteins that are required for self-replication.

Plasmid vectors include  

• pBR322  
• pUC18  
• F-plasmid