we boil the leaf in alcohol when we are testing it for starch.
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The hot water kills the leaf and the alcohol breaks down the chlorophyll, taking the green color out of the leaf. When you put iodine on the leaves, one of them will turn blue-black and the other will be a reddish-brown. Iodine is an indicator that turns blue-black in the presence of starch.
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To remove the green pigment present in the leaf we boil the leaf in alcohol when we are testing it for starch.
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