We have digested a circular plasmid dna with bamh i which has only one cutting site in the plasmid. The recognition site for bamh i is ggatcc. The digested product is incubated with pfu dna polymerase along with all the four dntps. Afterward the sample was treated with t4 dna ligase and the dna is purified. How many products will be there after ligation? Will the product be linear or circular? Can we digest the product again with bamhi?
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There will be 2 products nd the Product will be linear
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