Question

We use Safranin for staining-------------------- a. Cuticle of leaf b. Hypodermis of dicot stem c. Cortex of dicot root d. Mesophyll of dicot leaf​

Answer 1

We use safranin for staining a cuticle of the leaf.

• A plant consists of a thin layer on the epidermis of the leaf, it is called the cuticle.

• Cuticle is a protecting layer, it consists of cutin, a waxy, water repellent substance associated with suberin.

• Suberin is found in the cell walls of corky tissue. Safranine is an azo dye commonly used for plant microscopy, specially stain for lignified tissues.

• Safranine fluorescent is a staining technique used to stain the cuticle very nicely.

• This staining is used to detect the plant's cell structures and vascular tissues.

Answer 2

We use Safranin for staining cuticle of leaf.

Option: (a)

Explanation:  

• Safranin is a very important stain for staining plant and animal tissue. Saffranin can stain only those tissue which are very light and which are water permeable.  

• The cuticle of the leaf is the uppermost part which lies above the epidermis of leaf. It is a thin structure which is covered with flat epithelial cell.  

• On staining the cuticle of the leaf gives a characteristic brown to purple colour hands. It is useful in differentiating between monocot and dicot plants.

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