What 2 enzymes are used during DNA replication?
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In the prokaryotic system the following enzymes and proteins are involved in DNA replication:
DNA polymerase I, and III
Required for the addition of nucleotides to the 3′ OH of a complementary DNA strand.
DNA polymerase III has both 5 ‘→ 3’ and 3′→ 5′ proofreading activity. It has higher processivity and is holoenzyme with three core enzymes attached.
DNA polymerase I has both 5′→ 3′ proofreading activity. It is required to fill the gaps in the DNA after removal of Okazaki fragments.
It also removes the last RNA nucleotide of Okazaki fragment which is linked to DNA nucleotide and cannot be removed by RNaseH.
DnaA, DnaB (helicase), and DnaC (helicase loader)
DnaA is required for initiation of replication by unwinding the DNA strand at ori site.
DnaB is the helicase enzyme which is required for the separation of the strands.
DnaC is the helicase loader which loads the helicase enzyme on the DNA strand.
SSB (single strand binding) proteins
They bind the single-stranded portion of DNA which was unwound by helicase.
DNA primase ( a kind of RNA polymerase)
They are required for the primer synthesis. Without a primer, the DNA polymerase cannot initiate replication as it requires 3′ OH for the addition of nucleotide.
RNase H
This enzyme removes the RNA primers from the Okazaki fragments.
DNA ligase
It is required for the formation of a phosphodiester bond between the Okazaki fragments of the DNA.
Topoisomerase and Gyrase
They are required for supercoiling of the DNA (positive or negative supercoiling).
DNA polymerase I, and III
Required for the addition of nucleotides to the 3′ OH of a complementary DNA strand.
DNA polymerase III has both 5 ‘→ 3’ and 3′→ 5′ proofreading activity. It has higher processivity and is holoenzyme with three core enzymes attached.
DNA polymerase I has both 5′→ 3′ proofreading activity. It is required to fill the gaps in the DNA after removal of Okazaki fragments.
It also removes the last RNA nucleotide of Okazaki fragment which is linked to DNA nucleotide and cannot be removed by RNaseH.
DnaA, DnaB (helicase), and DnaC (helicase loader)
DnaA is required for initiation of replication by unwinding the DNA strand at ori site.
DnaB is the helicase enzyme which is required for the separation of the strands.
DnaC is the helicase loader which loads the helicase enzyme on the DNA strand.
SSB (single strand binding) proteins
They bind the single-stranded portion of DNA which was unwound by helicase.
DNA primase ( a kind of RNA polymerase)
They are required for the primer synthesis. Without a primer, the DNA polymerase cannot initiate replication as it requires 3′ OH for the addition of nucleotide.
RNase H
This enzyme removes the RNA primers from the Okazaki fragments.
DNA ligase
It is required for the formation of a phosphodiester bond between the Okazaki fragments of the DNA.
Topoisomerase and Gyrase
They are required for supercoiling of the DNA (positive or negative supercoiling).
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Hi mate here is ur answer.
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The enzymes used during DNA replication are
1.Helicase - Uses of hydrolysis of ATP to unzip or unwind DNA helix at the replication fork to allow the resulting single strands to be copied.
2. Primase - polymerises nucleotides triphosphates in a 5' to 3' directions exonuclease.
DNA primase and DNA polymerase are used during DNA replication.
DNA polymerase uses the RNA primer for the replication of single stranded DNA.
❇❇❇
The enzymes used during DNA replication are
1.Helicase - Uses of hydrolysis of ATP to unzip or unwind DNA helix at the replication fork to allow the resulting single strands to be copied.
2. Primase - polymerises nucleotides triphosphates in a 5' to 3' directions exonuclease.
DNA primase and DNA polymerase are used during DNA replication.
DNA polymerase uses the RNA primer for the replication of single stranded DNA.
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