Question

What are advantages and dis advantages of serial dilutions in serological test

Answer 1

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The serial dilution method involves a stepwise dilution of a substance in solution. Generally the dilution factor at each step is constant.

Advantages

1. It helps to reduce a dense culture of cells to a more usable concentration.

2. A specific amount of bacteria are reduced with every dilution.

3. The number of colonies cultured from serial dilutions of the sample are counted to estimate the concentration of an unknown sample. Then back track the measured counts to the unknown concentration.

Disadvantages

1. It does not separate bacteria like a streak plate.

2. Overnight incubation is required.