what are cloning vectors ?name any two cloning vectors?describe the features required to facilitate into cloning vectors?
Answers
A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.
Plasmid and Viruses are two cloning vectors
the features required to facilitate into cloning vectors;
Origin of Replication (Ori):
This is a sequence from where replication starts and any piece of DNA, when linked to this sequence can be made to replicate within the host cells.
This sequence is also responsible for controlling the copy number of the linked DNA.
Selectable Marker
It helps in identifying or selecting transformants and eliminating non-transformants; and selectively permits the growth of the transformants
Cloning (Recognition) Sites:
These are generally required to link the foreign or alien DNA with the vector. For this, the vector requires very few or single recognition sites for commonly used restriction enzymes. If more than one recognition sites is present within the vector, it will generate several fragments that will lead to more complication in gene cloning
Answer:
A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.
Plasmid and Viruses are two cloning vectors
the features required to facilitate into cloning vectors;
Origin of Replication (Ori):
This is a sequence from where replication starts and any piece of DNA, when linked to this sequence can be made to replicate within the host cells.
This sequence is also responsible for controlling the copy number of the linked DNA.
Selectable Marker
It helps in identifying or selecting transformants and eliminating non-transformants; and selectively permits the growth of the transformants
Cloning (Recognition) Sites:
These are generally required to link the foreign or alien DNA with the vector. For this, the vector requires very few or single recognition sites for commonly used restriction enzymes. If more than one recognition sites is present within the vector, it will generate several fragments that will lead to more complication in gene cloning
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Explanation: