what are effect of heat on RNA
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On heating the two strands of DNA separate out from each other (known as melting) and on cooling these strands again pair up/hybridize (called anealing).
The temperature at which the two stand separate completely is known as its melting temperature. The melting temperature of DNA depends upon the sequence of bases in the structure.
The structure having more content as compared to content has higher melting temperature. This is because base pair has three H-bonds, whereas pair has only two H-bonds. More H-bonds impart greater stability to the structure and has higher temperature.
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