What are restriction endonuclease?
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These are enzymes that cut a DNA at a specific specific recognition nucleotide sequence which is known as restriction.
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Restriction Endonuclease
》These are the enzymes which cuts the DNA strand at the ends.
》They cut the DNA only in the recognition sites.
》First of all, the enzyme suspects the entire strand of the DNA and once it find its recognition site, it binds to the DNA and cuts the two opposite strands.
》The recognition sites characterised by some base pairs are called the palindromic sequence.
》It cuts the DNA strand a little away from the palindromic sequence.
》They cut the two strands in the same base pairs thus resulting in sticky ends which are the overhanging structures.
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