Question

What are suspension culture? How are they initiated? Describe the various applications of suspension culture.

Answer 1

Suspension culture is a type of culture in which single cells or small aggregates of cells mul­tiply while suspended in agitated liquid medium. It is also referred to as cell culture or cell suspen­sion culture.

A suspension culture of higher plant cells consists of cells and cell clumps dispersed and growing in the agitated liquid medium. The most frequently added carbon sources (sucrose or glucose, 10–20 g/liter) are sufficient for the optimum growth of higher plant cell cultures, having a cell doubling time of 2–4 days. 

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Answer 2

Answer:

Answer:-

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The suspension cultures of single cells help in the understanding of the growth and developmental processes of a plant.

In the article, “Methods to isolate single cells”, all the methods of single-cell isolation have been discussed. Here, you will learn about different types of suspension culture for isolated single cells and how to assess the growth in these cultures.

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