what are the basic requirements of PCR technique ?
Answers
Answer:
The various components required for PCR include a DNA sample, DNA primers, free nucleotides called ddNTPs, and DNA polymerase. The various components required for PCR include a DNA sample, DNA primers, free nucleotides called ddNTPs, and DNA polymerase.
Answer:
The basic requirements of PCR Techniques are a DNA Strand, Primers, Four types of Deoxyribonucleotides and Taq Polymerase.
Explanation:
PCR stands for Polymerase Chain Reaction.
It is a technique used to make multiple copies of a given specific DNA region. This is carried out in-vitro.
The basic requirements into; ve-
DNA Strand is the one that has to be amplified.
Primers are oligonucleotides and are complementary to the DNA sequence.
The four deoxyribonucleotides are d ATP, d CTP,d TTP and d GTP.
Taq Polymerase is a DNA polymerase that can withstand high temperatures.
The process is carried out in three simple steps namely
1. Denaturation- It is done at a temperature of 96 degrees and provides a single strand template of DNA for annealing.
2. Annealing- This is basically to cool down the reaction so that primers can attach to their complementary sequence on template DNA
3. Extension- this is done at a temperature of 76 degrees and uses the Taq Polymerase which generates new strands of DNA by extending the primers.