What are the different types of plant tissue culture? describe the requirements for tissue culture?
Answers
Answered by
2
Plant Tissue Culture
Plant tissue culture was a new rendition to the methods of plant breeding that developed around the 1950s. Since the conventional breeding techniques could not fulfill the then required demand of crops, tissue culture came around as a grand leap in breeding practices. Plant tissue culture makes use of parts of a plant to generate multiple copies of the plant in a very short duration. The technique exploits the property of totipotency of plant cell which means that any cell from any part of the plant can be used to generate a whole new plant.
Types of Plant tissue culture
Seed Culture Embryo Culture Callus Culture Organ Culture Protoplast Culture Anther Culture
Procedure of Plant tissue culture
The part(s) of the plant used for culturing is known as explants. The explants are cultured in-vitro on a nutrient medium that caters to fulfill its nutritional requirements. The nutrient medium must provide the following:-
Macronutrients – This includes elements like nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S) which is required for proper growth and morphogenesis.Micronutrients – Elements like iron (Fe), manganese (Mn), zinc (Zn) etc., which are also crucial to the growth of tissues.Carbon or Energy source – This is one of the most crucial ingredients of the nutrient media. Sucrose is the most widely used carbon source among other carbohydrates that serve to provide C, H, and O.Vitamins, amino acids, and other inorganic salts.
Plant tissue culture was a new rendition to the methods of plant breeding that developed around the 1950s. Since the conventional breeding techniques could not fulfill the then required demand of crops, tissue culture came around as a grand leap in breeding practices. Plant tissue culture makes use of parts of a plant to generate multiple copies of the plant in a very short duration. The technique exploits the property of totipotency of plant cell which means that any cell from any part of the plant can be used to generate a whole new plant.
Types of Plant tissue culture
Seed Culture Embryo Culture Callus Culture Organ Culture Protoplast Culture Anther Culture
Procedure of Plant tissue culture
The part(s) of the plant used for culturing is known as explants. The explants are cultured in-vitro on a nutrient medium that caters to fulfill its nutritional requirements. The nutrient medium must provide the following:-
Macronutrients – This includes elements like nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S) which is required for proper growth and morphogenesis.Micronutrients – Elements like iron (Fe), manganese (Mn), zinc (Zn) etc., which are also crucial to the growth of tissues.Carbon or Energy source – This is one of the most crucial ingredients of the nutrient media. Sucrose is the most widely used carbon source among other carbohydrates that serve to provide C, H, and O.Vitamins, amino acids, and other inorganic salts.
Anny121:
if u like my answer then mark it as brainliest
Answered by
72
Question :-
What is Tissue culture ? Explain in detail.
Tissue culture :-
The method of growing a plant cell, tissue or an organ in an artificial nutrient medium under aseptic conditions provided with controlled conditions of light and temperature. Plants grown by micro propagation are genetically identical called soma clones, because they are produced by somatic cells.
Types of tissue culture are :-
◾ Seed Culture.
◾ Embryo Culture.
◾ Meristem Culture.
◾ Bud Culture.
◾ Callus Culture.
◾ Cell Suspension Culture.
◾ Anther Culture.
◾ Protoplast Culture.
Similar questions