What are the principal and application of chromatofraphy
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Chromatography’ is an analytical technique commonly used for separating a mixture of chemical substances into its individual components, so that the individual components can be thoroughly analyzed. There are many types of chromatography e.g., liquid chromatography, gas chromatography, ion-exchange chromatography, affinity chromatography, but all of these employ the same basic principles.
Chromatography plays an important role in many pharmaceutical industries and also in the chemical and food industry. Environmental testing laboratories generally want to identify for very small quantities of contaminants such as PCBs in waste oil, and pesticides. The Environmental Protection Agency makes the method of chromatography to test drinking water and to monitor air quality. Pharmaceutical industries use this method both to prepare huge quantities of extremely pure materials, and also to analyze the purified compounds for trace contaminants. The other applications of chromatography especially HPLC is used in Protein Separation like Insulin Purification, Plasma Fractionation and Enzyme Purification. These separation techniques like chromatography gain importance in different kinds of companies, different departments like Fuel Industry, biotechnology, biochemical processes, and forensic science. Chromatography is used for quality analyses and checker in the food industry, by identifying and separating, analyzing additives, vitamins, preservatives, proteins, and amino acids. Chromatography like HPLC is used in DNA fingerprinting and bioinformatics.