What are the principles behind the use of meristematic cells as virus free explants in micro propagation??
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Answer:
Answer:When plant breeders want to re-generate plants that are virus-free using asexual multiplication (in order to obtain clones of the primary unhealthy plant), they often collect the apical meristem from the infected plant, and cultivate it in appropriate media, so that a new, complete and virus-free plantlets develop. The fact that viruses spread systematically in the whole plant except the meristems when they colonize a new plant host, via phloem vascular element, is well known. But nothing is said about the putative molecular and cellular mechanisms that allow the pluripotent cells of the meristems to stay uninfected. Do you have some clues about this issue, or does this still remain a complete mystery of plant virology?
Answer:When plant breeders want to re-generate plants that are virus-free using asexual multiplication (in order to obtain clones of the primary unhealthy plant), they often collect the apical meristem from the infected plant, and cultivate it in appropriate media, so that a new, complete and virus-free plantlets develop. The fact that viruses spread systematically in the whole plant except the meristems when they colonize a new plant host, via phloem vascular element, is well known. But nothing is said about the putative molecular and cellular mechanisms that allow the pluripotent cells of the meristems to stay uninfected. Do you have some clues about this issue, or does this still remain a complete mystery of plant virology?Explanation:
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