Question

What are the requirements for PCR (Polymerase Chain Reaction)?

Answer 1

Initialization: This step is only required for DNA polymerases that require heat activation by hot-start PCR. It consists of heating the reaction chamber to atemperature of 94–96 °C (201–205 °F), or 98 °C (208 °F) if extremely thermostable polymerases are used, which is then held for 1–10 minutes.

Answer 2

❀ $\large{ \underline\textbf{Answer - }}$


This step is only required for DNA polymerases that require heat activation by hot-start PCR. It consists of heating the reaction chamber to atemperature of 94–96 °C (201–205 °F), or 98 °C (208 °F) if extremely thermostable polymerases are used, which is then held for 1–10 minutes.