What are the similarities between TLC and paper chromatography
Answers
Answer:Paper chromatography requires less preparation whereas thin layer chromatography requires more preparation time. 3. The stationary phase of paper chromatography is the water trapped in the cellulose filter paper. The stationary phase of the thin layer chromatography is the glass plates coated with silica gel.
Explanation:
Answer:
The feasibility of separating nucleic acid derivatives by thin-layer chromatography (TLC, Stahl, 1958, Stahl, 1961) has been recently demonstrated (Randerath, 1961a, Randerath, 1961b; Randerath and Struck, 1961). Instead of inorganic materials used preferentially in TLC1, ECTEOLA cellulose and cellulose layers were used in these investigations. In contrast to silica gel or alumina layers which show a very strong absorption in the 260 mμ region, ECTEOLA cellulose and cellulose chromatoplates can be examined like paper chromatograms under an ultraviolet lamp. Moreover, the separations of nucleotides are much better on ECTEOLA cellulose and cellulose layers than on inorganic adsorbents.
This communication is dealing with the comparison of paper chromatography (PC) with TLC of nucleic acid components on cellulose chromatoplates (cellulose layer chromatography, CLC).