Question

what are the steps in pcr cycle???
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Answer 1

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➡STEPS IN PCR CYCLE :::

❇DENATURATION

❇ANNEALING

❇EXTENSION

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Answer 2

Explanation:

The Polymerase chain reaction or PCR was originally invented by Kary Mullis in 1985 results in the selective amplification of the DNA (or RNA) present even as a single copy in initial preparation.

A single PCR amplification cycle involves three basic steps :-

The target DNA is heated to a high temperature (usually 94° to 98° C ) resulting in the separation of the two strand . Each strand of the target DNA then act as template for DNA synthesis .

The two oligonucleotide primers anneal (hybridize) to each of the single stranded DNA since sequence of the complementary to the 3' ends of the template DNA . This step is carried at a lower temperature (usually 40° to 60°) depending on the length and sequence of the primers .

Taq Polymerase (from a thermophilic bacteria Thermus aquaticus ) synthesis the DNA region between the primers, using DNTPs (deoxynucleoside triphosphates) and Mg2+ . It means the primers are extended towards eachother so that the DNA segment lying between the two primers is copied. This Occurs at an optimum temperature of 72°C .

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