Question

what are the steps involved in DNA finger printing

Answer 1

Procedure

• Gel electrophoresis is used to separate the fragments.

• The separated double stranded DNA fragments are treated with NaOH.

• As a result, double stranded DNA becomes single stranded DNA and denatured.

• The single stranded DNA are blotted on to nitrocellulose filter paper and then baked for 3 to 5 hours as a result the D fragments are fixed on the membrane.

• The nitrocellulose filter paper is placed in a solution containing DNA probe (Radio active labelled single stranded DNA).

• The DNA probe binds with the complementary sequences of the DNA fragments on the membrane to form a hybridised DNA.