What are the steps involved in isolating vinca alkaloid from periwinkle?
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Endophytes are the microbes which reside inside the plant, imitate the same mechanism of product formation as that of the host plant. In this work the endophytes from the plant Catharanthus roseus analysed for the production of vinca alkaloids as that of host which are potential anticancer agents. Endophytes were screened and six frequently growing endophytes were selected. These were subjected to two stage fermentation for production of alkaloids. Out of six endophytes, two endophytic fungi showed the presence of alkaloids by mayer’s and picric acid test. These culture filtrates were further analysed by the method HPLC (High Performance Liquid Chromatography). The two major anti-cancerous alkaloids vincristine and vinblastine were qualitatively determined by HPLC. The Rt values determined to be 11.58 and 21.72 for vinblastine and vincristine respectively