Question

What are the steps taken if an insulin-producing organism is to be developed?

Answer 1

The steps in the production of human insulin by genetic engineering method includes:

1. Human insulin is extracted from pancreas cells and an insulin-producing gene is isolated.

2. A plasmid DNA is extracted from a bacterium and cut with restriction enzyme, forming plasmid vector.

3. Insert human insulin-producing gene into the bacterial plasmid vector to form the recombinant DNA of human insulin-producing gene.

4. Introduce this recombinant DNA into a bacterial cell to form the recombinant bacterium.

5. The recombinant bacteria multiply in a fermentation tank and produce human insulin.

6. Insulin is extracted, purified and bottled. It is then ready to be injected into diabetic patients.

Answer 2

Human Insulin production steps are:-

1. Isolation of Donour or DNA segment
2. Formation of Recombinant DNA
3. Production of Multiple copies of rDNA.
4. Introduction of rDNA in recipient organisms.
5. Screening of Transformed cell.