Question

what are the three way to get gene of interest?
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Answer 1

Answer:

Your genes contain instructions that tell your cells to make molecules called proteins.

Answer 2

The three ways to get gene of interest are Isolation, Cloning

Explanation:

To get the gene of interest, the first step is to

1. Isolation of the gene of interest: The genes are present in long DNA molecules. Hence, one needs to choose an appropriate restriction endonuclease to cut the gene of interest from the whole DNA. Restriction endonuclease is enzymes that recognize a particular palindromic sequence. To isolate the gene of interest we use the gel electrophoresis technique.

2. Cloning: To clone the gene of interest we use Polymerase chain reaction (PCR).

3. Sequencing: The gene of interest can be sequenced by using Sanger method, Maxam Gilbert method or pyrosequencing.