what are the tools of rDNA ( recombinant DNA ) technology??
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Three main types of restriction endonucleases i.e., Type I, Type II and Type III are known with slightly different mode of action. Type II restriction enzymes are used in rDNA technology because they can be used in vitro to identify and cleave within specific DNA sequences usually having 4-8 nucleotides.
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The restriction enzymes – help to cut, the polymerases- help to synthesize and the ligases- help to bind. The restriction enzymes used in recombinant DNA technology play a major role in determining the location at which the desired gene is inserted into the vector genome.
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