Question

What can be the sitting sequence for restriction enzymes?

Answer 1

Explanation:

The 3' end of your restriction site will have the entire PCR product and will not need ... allow the restriction enzymes to sit over them before initiating the digestion reaction.

Answer 2

Answer:

The most common Type II enzymes are those like HhaI (NEB #R0139), HindIII (NEB #R0104), and NotI (NEB #R0189), that cleave DNA within their recognition sequences. Enzymes of this kind are the principal ones available commercially.