Question

what change occur in pyluric acid before entering mitochondria ??​

Answer 1

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In prokaryotic cells, the transition step occurs in the cytoplasm; in eukaryotic cells the pyruvates must first enter the mitochondria because the transition reaction and the citric acid cycle take place in the matrix of the mitochondria. The two molecules of acetyl-CoA can now enter the citric acid cycle.

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Answer 2

Pyruvic acid is first dehydrogenated to acetyl-CoA in the mitochondrion losing a carbon as CO2. This is the action of pyruvate dehydrogenase. Acetyl-CoA is then continuously pumped into the citric acid cycle (Figure 1.3.8). Basically, the 4-carbon keto acid oxaloacetic acid attaches to acetyl CoA to make the 6-carbon citric acid. The 6-carbon molecule loses 1 carbon as CO2becoming the 5-carbon keto acid α-ketoglutarate. A second carbon is then lost as CO2 making the 4-carbon structure succinyl-CoA. The remaining steps taking succinyl-CoA back to oxaloacetic acid involve resetting this basic keto acid (Figure 1.3.8). The citric acid cycle generates GTP, which is readily converted by its nucleoside-diphosphate kinase into ATP. The mitochondrial citric acid cycle is an amazing pathway efficiently cycling acetyl-CoA and very competently generating NADH for oxidative phosphorylation. The citric acid cycle is probably the central pathway to mitochondrial metabolism.





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