What DNS stands for Glucose estimation method
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3,5-dinitrosalicylic acid
Simultaneously, 3,5-dinitrosalicylic acid (DNS) is reduced to 3-amino-5-nitrosalicylic acid under alkaline conditions, as illustrated in the equation below :The chemistry of the reaction is complicated since standard curves do not always go through the origin and different sugars give different color yields.
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This involves the oxidation of the aldehyde functional group present in, for example, glucose and the ketone functional group in fructose. Simultaneously, 3,5-dinitrosalicylic acid (DNS) is reduced to 3-amino,5-nitrosalicylic acid under alkaline conditions: oxidation..
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