what do you observe whenthe leaf is spread in petridish and a few drops of tincture iodine / betadine solution is added on it? ( A.S-1 )
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The hot water kills the leaf and the alcohol breaks down the chlorophyll, taking the green color out of the leaf. When you put iodine on the leaves, one of them will turn blue-black and the other will be a reddish-brown. Iodine is an indicator that turns blue-black in the presence of starch. The leaf that was in the light turns blue-black, which demonstrates that the leaf has been performing photosynthesis and producing starch.
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