Question

What do you understand by colony PCR?

Answer 1

Colony PCR is a method used to screen for plasmids containing a desired insert directly from bacterial colonies without the need for culturing or plasmid purification steps. ... Pick a colony, "Poke" to a replica plate, and. Perform fast PCR using suspended E. coli cells as the template.

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Answer 2

Colony PCR is a method used to screen for plasmids containing a desired insert directly from bacterial colonies without the need for culturing or plasmid purification steps. (or)Colony PCR is a technique for rapidly screening bacterial colonies when subcloning plasmids or other DNA constructs. Basically, but designing a set of PCR primers that only amplify the desired plasmid, you can use PCR to determine in a bacterial colony has that construct.