Question

What does an optical part of the light microscope consist of?​

Answer 1

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The microscope optical train typically consists of an illuminator (including the light source and collector lens), a substage condenser, specimen, objective, eyepiece, and detector, which is either some form of camera or the observer's eye.

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Modern compound microscopes are designed to provide a magnified two-dimensional image that can be focused axially in successive focal planes, thus enabling a thorough examination of specimen fine structural detail in both two and three dimensions.

Most microscopes provide a translation mechanism attached to the stage that allows the microscopist to accurately position, orient, and focus the specimen to optimize visualization and recording of images. The intensity of illumination and orientation of light pathways throughout the microscope can be controlled with strategically placed diaphragms, mirrors, prisms, beamsplitters, and other optical elements to achieve the desired degree of brightness and contrast in the specimen.

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