Question

what does pcr stand for ? what is the principal of pcr ? name the different steps in pcr reaction?

Answer 1

PCR (polymerase chain reaction): PCR (polymerase chain reaction) is a technique in molecular genetics that permits the analysis of any short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA. PCR is used to reproduce (amplify) selected sections of DNA or RNA for analysis.

Answer 2

PCR [Polymerase Chain Reaction]

The principles of PCR: This method has in the field of molecular biology an irreplaceable role and constitutes one of the basic methods for DNA analysis.

Three different steps in PCR is:-

(1) Denaturing – when the double-stranded template DNA is heated to separate it into two single strands.

(2) Annealing – when the temperature is lowered to enable the DNA primers to attach to the template DNA.

(3) Extending – when the temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.