Question

what factor or factors are considered when a cell culture's environment is made as similar as possible to a cell's natural surroundings?

Answer 1

Cell culture is a process through which a cell is grown under controlled conditions which is often outside their natural environment.

The factors considered when a cell culture's environment is made similar as possible to a cell's natural surroundings are as follows;

1. An environment which is enriched with nutrients that supplies the cells with with vitamin, minerals and amino acids among other nutrients.

2. Various hormones with are  vital for cell's growth.

3. An environment which is enriched with gases such as carbon (IV) oxide and  oxygen.

4. Regulation of pH, temperature and atmospheric pressure.

Answer 2

The factors of a “cell culture's environment” is made as similar as possible to a “cell's natural surroundings” are:

i)    The cells are maintained and grown in a medium supplemented with glutamine, serum and antibiotics to maintain growth supplements and sterility and prevent growth of bacteria.

ii)    The pH of the growth medium is fixed to the pH of the body.

iii)   The cells are grown in an incubator where it is maintained at $\bold{37^{\circ} \mathrm{C}}$, humidity maintained and a fixed concentration of $\bold{\mathrm{CO}_{2}}.$