What factors are required for cloning?
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5.1. Unique and Subtle Structural Features of the Gene Sequence.
5.2. The “Strength” of the Transcriptional Promoter.
5.3.1. Reasons of Instability.
Nucleus
Egg Cell
A Surrogate female
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In the classical restriction enzyme digestion and ligation cloning protocols, cloning of any DNA fragment essentially involves four steps: isolation of the DNA of interest (or target DNA), ligation, transfection (or transformation), and
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